Judge Maksuda Khanom of additional district and session judge's court rejected the bail plea of Rana after taking hearing from both sides.
The judge fixed September 27 for recording deposition of the witnesses in the case.
Rana was taken to the court by a microbus from Kasimpur Central Jail in Gazipur at 10:30am.
Rana, now in jail, failed to get bail from the HC four times in the same case.
Awami League MP Amanur first appeared before the Tangail additional district and sessions judge on September 18 ending his 22-month fugitive run.
The AL leader went into ‘hiding’ after he was accused of killing freedom fighter and member of district unit AL Faruk Ahmed.
On January 18, 2013, the Awami League leader was shot dead in front of his residence in the district town. Later, Faruk’s wife Nahar Ahmed filed a case in this connection.
Fourteen people, including four brothers of Tangail’s influential Khan family, were accused in the murder case.