In Sylhet, MA Matin, 72, former civil surgeon of Moulvibazar, died at Shaheed Shamsuddin Ahmed Hospital around 9pm on Friday night.
He was hospitalised in the morning, said Sushanto Kumar Mohapatro, resident medical officer of the hospital.
His sample has been collected for coronavirus test.
Doctor Jahidul Islam, chief health officer of Sylhet City Corporation, said he was buried at Manikpir graveyard around 1am today following procedure.
In Khulna, Afzal Hossain, 70, who was undergoing treatment at isolation ward of Khulna Medical College Hospital with fever, cold related issues and breathing problem, died around 6am.
Confirming the matter, Doctor Shoilnedranath Biswas of the isolation ward, said that Afzal was admitted to the hospital on Thursday.
His sample has been collected for testing.
Bangladesh has seen a spike in the number of confirmed coronavirus cases and deaths in recent weeks.
The cases crossed the 30,000-mark on Friday with the detection of 1,694 new cases in the span of 24 hours. During this period, 24 more people died from COVID-19 disease, taking the death tally to 432.