Leaders and followers of the party formed the human chain around 10:15 am in front of the Jatiya Press Club 45 minutes before the scheduled time which ended at 12 noon.
The leaders and activists chanted different slogans demanding the release of Khaleda Zia immediately.
Huge number of law enforcers were deployed around the Jatiya Press Club to fend off any trouble.
BNP secretary general Mirza Fakhrul Islam Alamgir, its standing committee members Khandaker Mosharraf Hossain, Abdul Moyeen Khan and Nazrul Islam Khan addressed the programme.
BNP on Sunday got permission to stage a human chain programme in the city demanding Khaleda Zia's release from jail.
They will also observe a two-hour token hunger strike on Wednesday in all districts and metropolitan cities to press the same demand.
BNP chairperson Khaleda Zia has been in jail since a special court convicted her in Zia Orphanage Trust graft case.