Israel on Saturday intercepted a missile fired from Yemen, marking the first such attack from the country since the latest Middle East conflict began last month, raising concerns about the possible entry of Iran-backed Houthi rebels into the war.

The Houthis had earlier targeted vessels during the Israel-Hamas conflict, disrupting shipping routes in the Red Sea.

The development came despite signs of a possible breakthrough, as Tehran had agreed to allow humanitarian aid and agricultural shipments through the Strait of Hormuz.

Tensions escalated further after Israel struck Iran’s nuclear facilities hours after warning it would intensify its campaign against Tehran. In response, Iran launched an retaliatory attack on a Saudi air base, which U.S. officials said injured multiple American service members.

According to a source familiar with the matter, Iran fired six ballistic missiles and 29 drones at Prince Sultan Air Base in Saudi Arabia on Friday. At least 15 U.S. troops were wounded in the attack, including five seriously, though earlier reports had put the number at 10, with two critically injured.

Meanwhile, Oman reported a drone strike on Salalah port on Saturday morning that injured a foreign worker and damaged a crane.

In Bahrain, sirens were sounded ahead of a potential attack, with authorities urging residents to move to the nearest safe locations.