Though the identities of the deceased are yet to be established, police suspect they were family members.
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The accident occurred around 9.30 am in the highway's Zero Point area.
"The two were on their way to Khulna when the Satkhira-bound truck rammed their bike from behind. They fell off and came under the tyres of the truck," said officer-in-charge of Harintana police station Enamul Haque.
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The two men were rushed to a local hospital by passersby, where they were declared brought dead by doctors.
Frequent road fatalities
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Road accidents continue to spike in Bangladesh, with the Road Safety Foundation (RSF), an advocacy group for less dangerous roads, reporting 439 deaths from 417 accidents in November last year.
That meant fatalities were up nearly one-sixth or close to 16 percent from October, when 383 lives were lost in 314 accidents. The number of accidents also went up by nearly one-third, or 33 percent.
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Nirapad Sarak Chai, another road safety advocacy group, said in a report that 5,227 people were killed in 4,702 road accidents in Bangladesh last year.
The World Bank has said Bangladesh needs to invest an estimated extra $7.8 billion over the next decade to halve its fatalities from road crashes.
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The report pointed to the high death rate on Bangladesh’s roads as the consequence of a chronic lack of investment in systemic, targeted, and sustained road safety programmes.